DNA and cell biology
We have sequenced a genomic clone of the gene encoding the mouse mitochondrial DNA polymerase. The gene consists of 23 exons, which span approximately 13.2 kb, with exons ranging in size from 53 to 768 bp. All intron-exon boundaries conform to the GT-AG rule. By comparison with the human genomic sequence, we found remarkable conservation of the gene structure; the intron-exon borders are in almost identical locations for the 22 introns. The 5' upstream region contains approximately 300 bp of homology between the mouse and human sequences that presumably contain the promoter element. This region lacks any obvious TATA domain and is relatively GC rich, consistent with the housekeeping function of the mitochondrial DNA polymerase. Finally, within the 5' flanking region, both mouse and human genes have a region of 73 bp with high homology to the tRNA-Arg gene.
Animals, Base Sequence, DNA-Directed DNA Polymerase, Genes, Introns, Mice, Mitochondria, Molecular Sequence Data, Promoter Regions, Genetic, Sequence Alignment, Sequence Homology, Nucleic Acid
This is a copy of an article published in DNA and Cell Biology © 2000 copyright Mary Ann Liebert, Inc.; DNA and Cell Biology is available online at: http://online.liebertpub.com.
Mott, Justin L.; Denniger, Grace; Zullo, Steve J.; and Zassenhaus, H. Peter, "Genomic structure of murine mitochondrial DNA polymerase-gamma." (2000). Journal Articles: Biochemistry & Molecular Biology. 3.