Document Type

Article

Journal Title

Biochemical and biophysical research communications

Publication Date

6-10-2011

Volume

409

Abstract

Sialyl Lewis x (sLe(x)) plays an important role in cancer metastasis. But, the mechanism for its production in metastatic cancers remains unclear. The objective of current study was to examine the effects of a proinflammatory cytokine on the expression of glycosyltransferase and sulfotransferase genes involved in the synthesis of selectin ligands in a prostate cancer cell line. Androgen-independent human lymph node-derived metastatic prostate cancer cells (C-81 LNCaP), which express functional androgen receptor and mimic the castration-resistant advanced prostate cancer, were used. TNFα treatment of these cells increased their binding to P-, E- and L-selectins, anti-sLe(x) antibody, and anti-6-sulfo-sialyl Lewis x antibody by 12%, 240%, 43%, 248% and 21%, respectively. Also, the expression of C2GnT-1, B4GalT1, GlcNAc6ST3, and ST3Gal3 genes was significantly upregulated. Further treatment of TNFα-treated cells with either anti-sLe(x) antibody or E-selectin significantly suppressed their in vitro migration (81% and 52%, respectively) and invasion (45% and 56%, respectively). Our data indicate that TNFα treatment enhances the motility and invasion properties of LNCaP C-81 cells by increasing the formation of selectin ligands through stimulation of the expression of selective glycosyl- and sulfotransferase genes. These results support the hypothesis that inflammation contributes to cancer metastasis.

MeSH Headings

Cell Line, Tumor, Cell Movement, E-Selectin, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Glycosyltransferases, Humans, L-Selectin, Ligands, Male, Neoplasm Invasiveness, Oligosaccharides, P-Selectin, Prostatic Neoplasms, Sulfotransferases, Tumor Necrosis Factor-alpha

ISSN

1090-2104

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, [VOL#409, ISSUE#3, (2011)] 10.1016/j.bbrc.2011.05.019

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