Document Type


Journal Title

Neural plasticity

Publication Date

Winter 2-24-2015




Motor-skill learning induces changes in synaptic structure and function in the primary motor cortex through the involvement of a long-term potentiation- (LTP-) like mechanism. Although there is evidence that calcium-dependent release of gliotransmitters by astrocytes plays an important role in synaptic transmission and plasticity, the role of astrocytes in motor-skill learning is not known. To test the hypothesis that astrocytic activity is necessary for motor-skill learning, we perturbed astrocytic function using pharmacological and genetic approaches. We find that perturbation of astrocytes either by selectively attenuating IP3R2 mediated astrocyte Ca(2+) signaling or using an astrocyte specific metabolic inhibitor fluorocitrate (FC) results in impaired motor-skill learning of a forelimb reaching-task in mice. Moreover, the learning impairment caused by blocking astrocytic activity using FC was rescued by administration of the gliotransmitter D-serine. The learning impairments are likely caused by impaired LTP as FC blocked LTP in slices and prevented motor-skill training-induced increases in synaptic AMPA-type glutamate receptor in vivo. These results support the conclusion that normal astrocytic Ca(2+) signaling during a reaching task is necessary for motor-skill learning.

MeSH Headings

Animals, Antimetabolites, Astrocytes, Citrates, Estrogen Antagonists, Forelimb, In Vitro Techniques, Injections, Intraventricular, Inositol 1, 4, 5-Trisphosphate Receptors, Learning, Long-Term Potentiation, Mice, Mice, Inbred C57BL, Motor Skills, Mutation, Psychomotor Performance, Receptors, AMPA, Serine, Synaptic Transmission, Tamoxifen



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