Document Type
Article
Journal Title
Infection and Immunity
Publication Date
2025
Volume
93
Abstract
Biofilms are a cause of chronic, non-healing infections. Staphylococcus aureus is a proficient biofilm-forming pathogen commonly isolated from prosthetic joint infections that develop following primary arthroplasty. Extracellular adherence protein (Eap), previously characterized in planktonic or non-biofilm populations as being an adhesin and immune evasion factor, was recently identified in the exoproteome of S. aureus biofilms. This work demonstrates that Eap and its two functionally orphaned homologs EapH1 and EapH2 contribute to biofilm structure and prevent macrophage invasion and phagocytosis in these communities. Biofilms unable to express Eap proteins demonstrated increased porosity and reduced biomass. We describe the role of Eap proteins in vivo using a mouse model of S. aureus prosthetic joint infection. The Results suggest that the protection conferred to biofilms by Eap proteins is a function of biofilm structural stability that interferes with the leukocyte response to biofilm-associated bacteria.
MeSH Headings
Biofilms, Staphylococcus aureus, Animals, Prosthesis-Related Infections, Staphylococcal Infections, Mice, Bacterial Proteins, Bacterial Adhesion, Adhesins, Bacterial, Porosity, Macrophages, Disease Models, Animal, Phagocytosis
DOI Link
ISSN
1098-5522
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Bhattacharya, Mohini; Scherr, Tyler D.; Lister, Jessica; Kielian, Tammy; and Horswill, Alexander R., "Extracellular Adherence Proteins Reduce Matrix Porosity and Enhance Staphylococcus aureus Biofilm Survival during Prosthetic Joint Infection" (2025). Journal Articles: Pathology and Microbiology. 117.
https://digitalcommons.unmc.edu/com_pathmicro_articles/117