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Central States Society of Toxicology

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Preliminary studies suggest that the nitrosamine N-nitrosoatrazine (NNAT) is teratogenic and mutagenic. We hypothesized that the embryos exposed to NNAT would have higher mortality and lower growth rate compared to unexposed embryos. In this study, we evaluated growth and mortality in chick embryos after exposure to NNAT dissolved in dimethyl sulfoxide (DMSO). The study was divided into three experiments depending treatment design. First, the effect of DMSO on embryo growth and survival was tested. Second, we compared growth and survival between embryos treated with DMSO, 50:50 DMSO:water and NNAT at 0.245 µmol/l. Finally, we compared growth and survival between embryos treated with DMSO and varying doses of NNAT (1.11 µmol/l, 2.22 µmol/l, 3.33 µmol/l) dissolved in DMSO. Based on this, we determine the LD50 (lethal dose for 50% of a test population) for NNAT. In terms of mortality, the first experiment shows that there is no effect of DMSO compared to water and blank, but the third experiment shows that there is a linear relationship between NNAT doses where high NNAT dose level will reduce the survival rate of the embryos. From this relationship, we determine that LD50 to be 2. 85µmol/l. We continue the analysis on the survive embryos and reveals that DMSO and NNAT had no effect on the growth of embryos in all three experiments.


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Effect of N-nitrosoatrazine on Embryogenesis in Avian Embryos

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