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Pharmacology and Experimental Neuroscience
Background: Pericyte cells are an integral component of the vascular system and blood-brain barrier. HIV infection has been shown to impact both pericytes and the blood-brain barrier. Similarly, drugs of abuse have been found to alter blood-brain barrier permeability. Drugs of abuse and HIV infection comorbidity may affect pericyte function and viral replication.
Methods: Pericyte cells were treated with varying concentrations of either morphine, cocaine, or methamphetamine to determine cytotoxicity. Next, two concentrations were chosen and infected with macrophage tropic SHIV-BORI159N4. Viral supernatant was collected every three days for analysis viral titer using qPCR and other inflammatory markers.
Results: Drug treatment appeared to impact viral replication in pericyte cells. Most drug treatments produced lower viral titers, except for the methamphetamine at 10μM concentration treatment.
Conclusion: Drugs of abuse may impact how HIV infection affects pericyte cells, though underlying mechanisms are still not well-defined. Various classes of drugs may differentially alter viral replication within pericyte cells.
HIV, pericytes, macrophages, CNS, drugs of abuse
Li, Jennifer; Thurman, Michellie; Acharya, Arpan; and Byrareddy, Siddappa N., "Combined Effects of Drugs of Abuse and HIV Infection Comorbidity on Primary Pericytes" (2021). Posters: 2021 Summer Undergraduate Research Program. 31.