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Conference Proceeding

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Titanium (Ti) is the material of choice for orthopaedic applications because it is biocompatible and encourages osteoblast growth. The present study was devised to compare the osteoblast functions on nano-crystalline titanium oxide films produced by an ion beam assisted deposition (IBAD) technique with the osteoblast functions on micro-crystalline orthopaedic-grade titanium. To characterize the biocompatibility of these surfaces, we have studied cell adhesion, growth, and differentiation on different samples. Cell responses to surfaces were examined using the human osteosarcoma cell line (SAOS-2) with osteoblastic properties. To compare cell adhesion and growth, DAPI-stained nuclei were counted using ImageJ and Metamorph software. Immunofluorescence staining was also applied to monitor actin stress fiber shapes in order to compare cell morphology and actin remodeling at focal adhesion sites on the surfaces. Then, alizarin red assay was used to detect calcium compounds on surfaces which is an indication of successful in-vitro bone formation.

Our experimental results indicated that nan-crystalline TiO2 is superior to micro-crystalline Ti in supporting growth, adhesion, and proliferation. Also, significantly more calcium deposition was observed on the nano-surfaces.

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Orthopedics Commons