BACKGROUND: The use of immunodeficient mice transplanted with human hematopoietic stem cells is an accepted approach to study human-specific infectious diseases such as HIV-1 and to investigate multiple aspects of human immune system development. However, mouse and human are different in sialylation patterns of proteins due to evolutionary mutations of the CMP-N-acetylneuraminic acid hydroxylase (CMAH) gene that prevent formation of N-glycolylneuraminic acid from N-acetylneuraminic acid. How changes in the mouse glycoproteins' chemistry affect phenotype and function of transplanted human hematopoietic stem cells and mature human immune cells in the course of HIV-1 infection are not known.
RESULTS: We mutated mouse CMAH in the NOD/scid-IL2Rγ
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Dagur, Raghubendra S.; Branch-Woods, Amanda; Mathews, Saumi; Joshi, Poonam S.; Quadros, Rolen M.; Harms, Donald W.; Cheng, Yan; Miles, Shana M.; Pirruccello, Samuel J.; Gurumurthy, Channabasavaiah B.; Gorantla, Santhi; and Poluektova, Larisa Y., "Human-like NSG Mouse Glycoproteins Sialylation Pattern Changes the Phenotype of Human Lymphocytes and Sensitivity to HIV-1 Infection" (2019). Journal Articles: Munroe-Meyer Institute. 4.