Document Type

Article

Journal Title

British journal of cancer

Publication Date

3-15-2011

Volume

104

Abstract

BACKGROUND: Pancreatic cancer (PC) harbours an activated point mutation (Kras(G12D)) in the Kras proto-oncogene that has been demonstrated to promote the development of PC.

METHODS: This study was designed to investigate the effect of the oncogenic Kras(G12D) allele on aggressiveness and metastatic potential of PC cells. We silenced the oncogenic Kras(G12D) allele expression in CD18/HPAF and ASPC1 cell lines by stable expression of shRNA specific to the Kras(G12D)allele.

RESULTS: The Kras(G12D) knockdown cells exhibited a significant decrease in motility (P

CONCLUSIONS: The results of this study suggest that the Kras(G12D) allele promotes metastasis in PC cells partly through the downregulation of E-cadherin.

MeSH Headings

Adenocarcinoma, Amino Acid Substitution, Animals, Aspartic Acid, Cadherins, Down-Regulation, Gene Expression Regulation, Neoplastic, Glycine, Humans, Mice, Mice, Nude, Neoplasm Invasiveness, Neoplasm Metastasis, Pancreatic Neoplasms, Point Mutation, Proto-Oncogene Proteins, RNA, Small Interfering, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, ras Proteins

ISSN

1532-1827

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

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