British journal of cancer
BACKGROUND: Pancreatic cancer (PC) harbours an activated point mutation (Kras(G12D)) in the Kras proto-oncogene that has been demonstrated to promote the development of PC.
METHODS: This study was designed to investigate the effect of the oncogenic Kras(G12D) allele on aggressiveness and metastatic potential of PC cells. We silenced the oncogenic Kras(G12D) allele expression in CD18/HPAF and ASPC1 cell lines by stable expression of shRNA specific to the Kras(G12D)allele.
RESULTS: The Kras(G12D) knockdown cells exhibited a significant decrease in motility (P
CONCLUSIONS: The results of this study suggest that the Kras(G12D) allele promotes metastasis in PC cells partly through the downregulation of E-cadherin.
Adenocarcinoma, Amino Acid Substitution, Animals, Aspartic Acid, Cadherins, Down-Regulation, Gene Expression Regulation, Neoplastic, Glycine, Humans, Mice, Mice, Nude, Neoplasm Invasiveness, Neoplasm Metastasis, Pancreatic Neoplasms, Point Mutation, Proto-Oncogene Proteins, RNA, Small Interfering, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, ras Proteins
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Rachagani, Satyanarayana; Senapati, S; Chakraborty, S; Ponnusamy, Moorthy P.; Kumar, Sushil; Smith, Lynette; Jain, Maneesh; and Batra, Surinder K., "Activated KrasG¹²D is associated with invasion and metastasis of pancreatic cancer cells through inhibition of E-cadherin." (2011). Journal Articles: Biochemistry & Molecular Biology. 82.