DigitalCommons@UNMC

Title

Activated KrasG¹²D is associated with invasion and metastasis of pancreatic cancer cells through inhibition of E-cadherin.

Authors

Satyanarayana Rachagani, University of Nebraska Medical CenterFollow
S Senapati, University of Nebraska Medical Center
S Chakraborty, University of Nebraska Medical Center
Moorthy P. Ponnusamy, University of Nebraska Medical CenterFollow
Sushil Kumar, University of Nebraska Medical CenterFollow
Lynette Smith, University of Nebraska Medical CenterFollow
Maneesh Jain, University of Nebraska Medical CenterFollow
Surinder K. Batra, University of Nebraska Medical CenterFollow

Document Type

Article

Journal Title

British journal of cancer

Publication Date

3-15-2011

Volume

104

Abstract

BACKGROUND: Pancreatic cancer (PC) harbours an activated point mutation (Kras(G12D)) in the Kras proto-oncogene that has been demonstrated to promote the development of PC.

METHODS: This study was designed to investigate the effect of the oncogenic Kras(G12D) allele on aggressiveness and metastatic potential of PC cells. We silenced the oncogenic Kras(G12D) allele expression in CD18/HPAF and ASPC1 cell lines by stable expression of shRNA specific to the Kras(G12D)allele.

RESULTS: The Kras(G12D) knockdown cells exhibited a significant decrease in motility (P

CONCLUSIONS: The results of this study suggest that the Kras(G12D) allele promotes metastasis in PC cells partly through the downregulation of E-cadherin.

MeSH Headings

Adenocarcinoma, Amino Acid Substitution, Animals, Aspartic Acid, Cadherins, Down-Regulation, Gene Expression Regulation, Neoplastic, Glycine, Humans, Mice, Mice, Nude, Neoplasm Invasiveness, Neoplasm Metastasis, Pancreatic Neoplasms, Point Mutation, Proto-Oncogene Proteins, RNA, Small Interfering, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, ras Proteins

ISSN

1532-1827

DOI Link

10.1038/bjc.2011.31

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

Recommended Citation

Rachagani, Satyanarayana; Senapati, S; Chakraborty, S; Ponnusamy, Moorthy P.; Kumar, Sushil; Smith, Lynette; Jain, Maneesh; and Batra, Surinder K., "Activated KrasG¹²D is associated with invasion and metastasis of pancreatic cancer cells through inhibition of E-cadherin." (2011). Journal Articles: Biochemistry & Molecular Biology. 82.
https://digitalcommons.unmc.edu/com_bio_articles/82