Document Type
Article
Journal Title
Bioscience Reports
Publication Date
10-29-2021
Volume
41
Abstract
The role of human prostatic acid phosphatase (PAcP, P15309|PPAP_HUMAN) in prostate cancer was investigated using a new proteomics tool termed signal sequence swapping (replacement of domains from the native cleaved amino terminal signal sequence of secretory/membrane proteins with corresponding regions of functionally distinct signal sequence subtypes). This manipulation preferentially redirects proteins to different pathways of biogenesis at the endoplasmic reticulum (ER), magnifying normally difficult to detect subsets of the protein of interest. For PAcP, this technique reveals three forms identical in amino acid sequence but profoundly different in physiological functions, subcellular location, and biochemical properties. These three forms of PAcP can also occur with the wildtype PAcP signal sequence. Clinical specimens from patients with prostate cancer demonstrate that one form, termed PLPAcP, correlates with early prostate cancer. These findings confirm the analytical power of this method, implicate PLPAcP in prostate cancer pathogenesis, and suggest novel anticancer therapeutic strategies.
ISSN
1573-4935
DOI Link
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Alpert, Evgenia; Akhavan, Armin; Gruzman, Arie; Hansen, William J.; Lehrer-Graiwer, Joshua; Hall, Steven C.; Johansen, Eric; McAllister, Sean; Gulati, Mittul; Lin, Ming-Fong; and Lingappa, Vishwanath R, "Multifunctionality of Prostatic Acid Phosphatase in Prostate Cancer Pathogenesis" (2021). Journal Articles: Biochemistry & Molecular Biology. 145.
https://digitalcommons.unmc.edu/com_bio_articles/145