Document Type

Article

Journal Title

PLoS One

Publication Date

Fall 10-25-2013

Volume

8

Abstract

Transmembrane mucins, MUC4 and MUC16 are associated with tumor progression and metastatic potential in human pancreatic adenocarcinoma. We discovered that miR-200c interacts with specific sequences within the coding sequence of MUC4 and MUC16 mRNAs, and evaluated the regulatory nature of this association. Pancreatic cancer cell lines S2.028 and T3M-4 transfected with miR-200c showed a 4.18 and 8.50 fold down regulation of MUC4 mRNA, and 4.68 and 4.82 fold down regulation of MUC16 mRNA compared to mock-transfected cells, respectively. A significant reduction of glycoprotein expression was also observed. These results indicate that miR-200c overexpression regulates MUC4 and MUC16 mucins in pancreatic cancer cells by directly targeting the mRNA coding sequence of each, resulting in reduced levels of MUC4 and MUC16 mRNA and protein. These data suggest that, in addition to regulating proteins that modulate EMT, miR-200c influences expression of cell surface mucins in pancreatic cancer.

MeSH Headings

Base Pairing, Base Sequence, Binding Sites, CA-125 Antigen, Cell Line, Tumor, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Gene Order, Humans, Membrane Proteins, MicroRNAs, Mucin-4, Open Reading Frames, Pancreatic Neoplasms, RNA Interference

ISSN

1932-6203

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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