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Title

Impaired expression of protein phosphatase 2A subunits enhances metastatic potential of human prostate cancer cells through activation of AKT pathway.

Authors

P Pandey, University of Nebraska Medical CenterFollow
Parthasarathy Seshacharyulu, University of Nebraska Medical CenterFollow
Srustidhar Das, University of Nebraska Medical CenterFollow
Satyanarayana Rachagani, University of Nebraska Medical CenterFollow
Moorthy P. Ponnusamy, University of Nebraska Medical CenterFollow
Y Yan, University of Nebraska Medical CenterFollow
Sonny L. Johansson, University of Nebraska Medical CenterFollow
K Datta, University of Nebraska Medical CenterFollow
Ming-Fong Lin, University of Nebraska Medical CenterFollow
Surinder K. Batra, University of Nebraska Medical CenterFollow

Document Type

Article

Journal Title

British journal of cancer

Publication Date

6-25-2013

Volume

108

Abstract

BACKGROUND: Protein phosphatase 2A (PP2A) is a dephosphorylating enzyme, loss of which can contribute to prostate cancer (PCa) pathogenesis. The aim of this study was to analyse the transcriptional and translational expression patterns of individual subunits of the PP2A holoenzyme during PCa progression.

METHODS: Immunohistochemistry (IHC), western blot, and real-time PCR was performed on androgen-dependent (AD) and androgen-independent (AI) PCa cells, and benign and malignant prostate tissues for all the three PP2A (scaffold, regulatory, and catalytic) subunits. Mechanistic and functional studies were performed using various biochemical and cellular techniques.

RESULTS: Through immunohistochemical analysis we observed significantly reduced levels of PP2A-A and -B'γ subunits (P

CONCLUSION: We conclude that loss of expression of scaffold and regulatory subunits of PP2A is responsible for its altered function during PCa pathogenesis.

MeSH Headings

Adenocarcinoma, Cell Line, Tumor, Disease Progression, Down-Regulation, Enzyme Activation, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Humans, Male, Models, Biological, Neoplasm Metastasis, Oncogene Protein v-akt, Prostatic Neoplasms, Protein Phosphatase 2, Protein Subunits, Signal Transduction

ISSN

1532-1827

DOI Link

10.1038/bjc.2013.160

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

Recommended Citation

Pandey, P; Seshacharyulu, Parthasarathy; Das, Srustidhar; Rachagani, Satyanarayana; Ponnusamy, Moorthy P.; Yan, Y; Johansson, Sonny L.; Datta, K; Lin, Ming-Fong; and Batra, Surinder K., "Impaired expression of protein phosphatase 2A subunits enhances metastatic potential of human prostate cancer cells through activation of AKT pathway." (2013). Journal Articles: Biochemistry & Molecular Biology. 79.
https://digitalcommons.unmc.edu/com_bio_articles/79