Document Type
Article
Journal Title
Respiratory Research
Publication Date
3-7-2019
Volume
20
Abstract
Agriculture exposures are associated with reducing the risk of allergy and asthma in early life; yet, repeated exposures later in life are associated with chronic bronchitis and obstructive pulmonary diseases. The objective of this study was to investigate the airway inflammatory response to organic dust extract (ODE) in mice with established ovalbumin (OVA)-induced experimental asthma. C57BL/6 mice were either OVA sensitized/aerosol-exposed or saline (Sal) sensitized/aerosol-challenged. Both groups were then subsequently challenged once with intranasal saline or swine confinement ODE to obtain 4 treatment groups of Sal-Sal, Sal-ODE, OVA-Sal, and OVA-ODE. Airway hyper-responsiveness (AHR) to methacholine, bronchiolar lavage fluid, lung tissues, and serum were collected. Intranasal inhalation of ODE in OVA-treated (asthmatic) mice (OVA-ODE) increased AHR and total cellular influx marked by elevated neutrophil and eosinophil counts. Flow cytometry analysis further demonstrated that populations of CD11c
DOI Link
ISSN
1465-993X
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Warren, Kristi J.; Dickinson, John D.; Nelson, Amy J.; Wyatt, Todd A.; Romberger, Debra J.; and Poole, Jill A., "Ovalbumin-Sensitized Mice Have Altered Airway Inflammation to Agriculture Organic Dust" (2019). Journal Articles: Internal Medicine. 10.
https://digitalcommons.unmc.edu/com_internal_articles/10