A Role for B Cells in Organic Dust Induced Lung Inflammation

Authors

Jill A. Poole, University of Nebraska Medical CenterFollow
Ted R. Mikuls, University of Nebraska Medical CenterFollow
Michael J. Duryee, University of Nebraska Medical CenterFollow
Kristi J. Warren, University of Nebraska Medical Center
Todd A. Wyatt, twyatt@unmc.eduFollow
Amy J. Nelson, University of Nebraska Medical CenterFollow
Debra J. Romberger, University of Nebraska Medical CenterFollow
William W. West, University of Nebraska Medical Center
Geoffrey M. Thiele, University of Nebraska Medical CenterFollow

Document Type

Article

Journal Title

Respiratory Research

Publication Date

2017

Volume

18

Abstract

BACKGROUND: Agriculture organic dust exposures induce lung disease with lymphoid aggregates comprised of both T and B cells. The precise role of B cells in mediating lung inflammation is unknown, yet might be relevant given the emerging role of B cells in obstructive pulmonary disease and associated autoimmunity.

METHODS: Using an established animal model, C57BL/6 wild-type (WT) and B-cell receptor (BCR) knock-out (KO) mice were repetitively treated with intranasal inhalation of swine confinement organic dust extract (ODE) daily for 3 weeks and lavage fluid, lung tissues, and serum were collected.

RESULTS: ODE-induced neutrophil influx in lavage fluid was not reduced in BCR KO animals, but there was reduction in TNF-α, IL-6, CXCL1, and CXCL2 release. ODE-induced lymphoid aggregates failed to develop in BCR KO mice. There was a decrease in ODE-induced lung tissue CD11c+CD11b+ exudative macrophages and compensatory increase in CD8+ T cells in lavage fluid of BCR KO animals. Compared to saline, there was an expansion of conventional B2-, innate B1 (CD19+CD11b+CD5+/-)-, and memory (CD19+CD273+/-CD73+/-) B cells following ODE exposure in WT mice. Autoreactive responses including serum IgG anti-citrullinated protein antibody (ACPA) and anti-malondialdehyde-acetaldehyde (MAA) autoantibodies were increased in ODE treated WT mice as compared to saline control. B cells and serum immunoglobulins were not detected in BCR KO animals.

CONCLUSIONS: Lung tissue staining for citrullinated and MAA modified proteins were increased in ODE-treated WT animals, but not BCR KO mice. These studies show that agriculture organic dust induced lung inflammation is dependent upon B cells, and dust exposure induces an autoreactive response.

DOI Link

10.1186/s12931-017-0703-x

ISSN

1465-993X

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Poole, Jill A.; Mikuls, Ted R.; Duryee, Michael J.; Warren, Kristi J.; Wyatt, Todd A.; Nelson, Amy J.; Romberger, Debra J.; West, William W.; and Thiele, Geoffrey M., "A Role for B Cells in Organic Dust Induced Lung Inflammation" (2017). Journal Articles: Internal Medicine. 6.
https://digitalcommons.unmc.edu/com_internal_articles/6