Molecular diagnosis of Burkitt's lymphoma.

Authors

• Sandeep S. Dave, National Institutes of Health, Bethesda, MD
• Kai Fu, University of Nebraska Medical CenterFollow
• George W. Wright, National Institutes of Health, Bethesda, MD
• Lloyd T. Lam, National Institutes of Health, Bethesda, MD
• Philip Kluin, University of Groningen
• Evert-Jan Boerma, University of Groningen
• Timothy Greiner, University of Nebraska Medical CenterFollow
• Dennis D. Weisenburger, University of Nebraska Medical Center
• Andreas Rosenwald, University of Würzburg
• German Ott, University of Würzburg
• Hans-Konrad Müller-Hermelink, University of Würzburg
• Randy D. Gascoyne, British Columbia Cancer Agency
• Jan Delabie, Norway Hospital Clinic
• Lisa M. Rimsza, Southwest Oncology Group
• Rita M. Braziel, Southwest Oncology Group
• Thomas M. Grogan, Southwest Oncology Group
• Elias Campo, University of Barcelona
• Elaine S. Jaffe, National Institutes of Health, Bethesda, MD
• Bhavana J. Dave, University of Nebraska Medical CenterFollow
• Warren Sanger, University of Nebraska Medical Center
• M Bast, University of Nebraska Medical CenterFollow
• Julie M. Vose, University of Nebraska Medical CenterFollow
• James O. Armitage, University of Nebraska Medical CenterFollow
• Joseph M. Connors, British Columbia Cancer Agency
• Erlend B. Smeland, Norway Hospital Clinic
• Stein Kvaloy, Norway Hospital Clinic
• Harald Holte, Norway Hospital Clinic
• Richard I. Fisher, Southwest Oncology Group
• Thomas P. Miller, Southwest Oncology Group
• Emilio Montserrat, University of Barcelona
• Wyndham H. Wilson, National Institutes of Health, Bethesda, MD
• Manisha Bahl, National Institutes of Health, Bethesda, MD
• Hong Zhao, National Institutes of Health, Bethesda, MD
• Liming Yang, National Institutes of Health, Bethesda, MD
• John Powell, National Institutes of Health, Bethesda, MD
• Richard Simon, National Institutes of Health, Bethesda, MD
• Wing C. Chan, University of Nebraska Medical Center
• Louis M. Staudt, National Institutes of Health, Bethesda, MD

Document Type

Article

Journal Title

The New England journal of medicine

Publication Date

Summer 6-8-2006

Volume

354

Abstract

BACKGROUND: The distinction between Burkitt's lymphoma and diffuse large-B-cell lymphoma is crucial because these two types of lymphoma require different treatments. We examined whether gene-expression profiling could reliably distinguish Burkitt's lymphoma from diffuse large-B-cell lymphoma.

METHODS: Tumor-biopsy specimens from 303 patients with aggressive lymphomas were profiled for gene expression and were also classified according to morphology, immunohistochemistry, and detection of the t(8;14) c-myc translocation.

RESULTS: A classifier based on gene expression correctly identified all 25 pathologically verified cases of classic Burkitt's lymphoma. Burkitt's lymphoma was readily distinguished from diffuse large-B-cell lymphoma by the high level of expression of c-myc target genes, the expression of a subgroup of germinal-center B-cell genes, and the low level of expression of major-histocompatibility-complex class I genes and nuclear factor-kappaB target genes. Eight specimens with a pathological diagnosis of diffuse large-B-cell lymphoma had the typical gene-expression profile of Burkitt's lymphoma, suggesting they represent cases of Burkitt's lymphoma that are difficult to diagnose by current methods. Among 28 of the patients with a molecular diagnosis of Burkitt's lymphoma, the overall survival was superior among those who had received intensive chemotherapy regimens instead of lower-dose regimens.

CONCLUSIONS: Gene-expression profiling is an accurate, quantitative method for distinguishing Burkitt's lymphoma from diffuse large-B-cell lymphoma.

MeSH Headings

Adolescent, Adult, Aged, Antineoplastic Combined Chemotherapy Protocols, Bayes Theorem, Burkitt Lymphoma, Child, Child, Preschool, Diagnosis, Differential, Female, Follow-Up Studies, Gene Expression, Gene Expression Profiling, Genes, MHC Class I, Genes, myc, Humans, In Situ Hybridization, Fluorescence, Lymphoma, B-Cell, Male, Middle Aged, NF-kappa B, Oligonucleotide Array Sequence Analysis, Survival Analysis, Transcription, Genetic, Translocation, Genetic

DOI Link

10.1056/NEJMoa055759

ISSN

1533-4406

Rights

From the New England Journal of Medicine, Dave SS, Fu K, Wright GW, et al., Molecular diagnosis of Burkitt's lymphoma, 354, 2431-2442 . Copyright © (2006) Massachusetts Medical Society. Reprinted with permission.

Recommended Citation

Dave, Sandeep S.; Fu, Kai; Wright, George W.; Lam, Lloyd T.; Kluin, Philip; Boerma, Evert-Jan; Greiner, Timothy; Weisenburger, Dennis D.; Rosenwald, Andreas; Ott, German; Müller-Hermelink, Hans-Konrad; Gascoyne, Randy D.; Delabie, Jan; Rimsza, Lisa M.; Braziel, Rita M.; Grogan, Thomas M.; Campo, Elias; Jaffe, Elaine S.; Dave, Bhavana J.; Sanger, Warren; Bast, M; Vose, Julie M.; Armitage, James O.; Connors, Joseph M.; Smeland, Erlend B.; Kvaloy, Stein; Holte, Harald; Fisher, Richard I.; Miller, Thomas P.; Montserrat, Emilio; Wilson, Wyndham H.; Bahl, Manisha; Zhao, Hong; Yang, Liming; Powell, John; Simon, Richard; Chan, Wing C.; and Staudt, Louis M., "Molecular diagnosis of Burkitt's lymphoma." (2006). Journal Articles: Pathology and Microbiology. 33.
https://digitalcommons.unmc.edu/com_pathmicro_articles/33