Document Type
Article
Journal Title
Scientific Reports
Publication Date
Winter 1-1-2013
Volume
3
Abstract
Recent reports have demonstrated that somatic cells can be directly converted to other differentiated cell types through ectopic expression of sets of transcription factors, directly avoiding the transition through a pluripotent state. Our previous experiments generated induced neural progenitor-like cells (iNPCs) by a novel combination of five transcription factors (Sox2, Brn2, TLX, Bmi1 and c-Myc). Here we demonstrated that the iNPCs not only possess NPC-specific marker genes, but also have qualities of primary brain-derived NPCs (WT-NPCs), including tripotent differentiation potential, mature neuron differentiation capability and synapse formation. Importantly, the mature neurons derived from iNPCs exhibit significant physiological properties, such as potassium channel activity and generation of action potential-like spikes. These results suggest that directly reprogrammed iNPCs closely resemble WT-NPCs, which may suggest an alternative strategy to overcome the restricted proliferative and lineage potential of induced neurons (iNCs) and broaden applications of cell therapy in the treatment of neurodegenerative disorders.
MeSH Headings
Animals, Cell Differentiation, Fibroblasts, Gene Expression Profiling, Mice, Neural Stem Cells, Neurons, Synapses, Synaptic Potentials, Transcription Factors
DOI Link
ISSN
2045-2322
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Tian, Changhai; Liu, Qiang; Ma, Kangmu; Wang, Yongxiang; Chen, Qiang; Ambroz, Randall; Klinkebiel, David L.; Li, Yuju; Huang, Yunlong; Ding, Jianqing; Wu, Jie; and Zheng, Jialin C., "Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors." (2013). Journal Articles: Pharmacology & Experimental Neuroscience. 46.
https://digitalcommons.unmc.edu/com_pen_articles/46