Document Type
Article
Journal Title
Scientific Reports
Publication Date
2021
Volume
11
Abstract
Congenital aortic valve stenosis (CAVS) affects up to 10% of the world population without medical therapies to treat the disease. New molecular targets are continually being sought that can halt CAVS progression. Collagen deregulation is a hallmark of CAVS yet remains mostly undefined. Here, histological studies were paired with high resolution accurate mass (HRAM) collagen-targeting proteomics to investigate collagen fiber production with collagen regulation associated with human AV development and pediatric end-stage CAVS (pCAVS). Histological studies identified collagen fiber realignment and unique regions of high-density collagen in pCAVS. Proteomic analysis reported specific collagen peptides are modified by hydroxylated prolines (HYP), a post-translational modification critical to stabilizing the collagen triple helix. Quantitative data analysis reported significant regulation of collagen HYP sites across patient categories. Non-collagen type ECM proteins identified (26 of the 44 total proteins) have direct interactions in collagen synthesis, regulation, or modification. Network analysis identified BAMBI (BMP and Activin Membrane Bound Inhibitor) as a potential upstream regulator of the collagen interactome. This is the first study to detail the collagen types and HYP modifications associated with human AV development and pCAVS. We anticipate that this study will inform new therapeutic avenues that inhibit valvular degradation in pCAVS and engineered options for valve replacement.
MeSH Headings
Adolescent, Aortic Valve, Aortic Valve Stenosis, Child, Child, Preschool, Collagen, Female, Heart Defects, Congenital, Humans, Hydroxylation, Infant, Infant, Newborn, Male, Protein Processing, Post-Translational, Proteomics
DOI Link
ISSN
2045-2322
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Clift, Cassandra L.; Su, Yan Ru; Bichell, David; Jensen Smith, Heather; Bethard, Jennifer R.; Norris-Caneda, Kim; Comte-Walters, Susana; Ball, Lauren E.; Hollingsworth, Michael A.; Mehta, Anand S.; Drake, Richard R.; and Angel, Peggi M., "Collagen Fiber Regulation in Human Pediatric Aortic Valve Development and Disease" (2021). Journal Articles: Eppley Institute. 43.
https://digitalcommons.unmc.edu/eppley_articles/43