Graduation Date

Summer 8-19-2016

Document Type


Degree Name

Master of Science (MS)


Medical Sciences Interdepartmental Area

First Advisor

Richard A. Reinhardt, DDS, PhD

Second Advisor

Thomas M. Petro, PhD

Third Advisor

Eric Y.K. Fung, PhD


The objectives of this study were to compare the effect of SRP+MM versus SRP alone on inflammatory cytokines, interleukin (IL)-1β and IL-1 receptor antagonist (ra), found in gingival crevicular fluid (GCF) and saliva, and correlate IL-1 values with clinical outcomes over a 2-year period of PMT. Fifty-five patients with an initial posterior pocket ≥5 mm and history of bleeding on probing (BOP) were randomized to test (SRP+MM, n=27) and control (SRP, n=28) groups. Clinical measurements and GCF samples were done at baseline and 24 months. SRP+MM or SRP were provided at 6-month intervals. IL-1β and IL-1ra were measured in GCF and saliva (24 months). A total of 48 patients completed the study (SRP+MM, n=23; SRP, n=25). Groups were compared using t-test, Wilcoxon test, and Spearman correlations. There was no significant difference or change in IL-1 or IL-1β/IL-1ra ratios levels between groups after 24 months. BOP level decreased significantly in both groups (SRP+MM=56%, SRP=45%). Bone loss was not significantly altered in either group. Both groups showed significant improvements in probing depth (p=0.001) and clinical attachment level (p=0.001). Baseline GCF IL-1β was correlated with bone loss at 24 months and salivary IL-1β was correlated with increase in probing depth at 24 months (r=0.34, p≤0.03). The addition of MM to SRP during PMT did not significantly affect IL-1 levels or clinical parameters.