DigitalCommons@UNMC

Title

Using RT-PCR to Determine the Presence of Various Viruses Found in Nebraska Wastewater

Author

Gabrielle H. Estep, Northwest Missouri State UniversityFollow
Ashley Peer, University of Nebraska Medical CenterFollow
Mohammad Salimi, University of Nebraska at Omaha
Michael Wiley, University of Nebraska Medical Center
Catherine Pratt, University of Nebraska Medical Center

Files

Presentation date

Summer 2022

College, Institute, or Department

College of Public Health

Research Mentor

Catherine Pratt

Abstract

Detection of Adenovirus, Aichi virus, Influenza, Cytomegalovirus, respiratory syncytial virus, and Hepatitis A in Nebraska wastewater using RT-PCR. Extraction of virus nucleic acid was done using Dynabead enrichment and MagMAX wastewater extraction kit.

Keywords

wastewater, extraction, viruses, RT-PCR, q-PCR, dynabeads, aichi virus, adenovirus, influenza, cmv, rsv, Hepatitis A, surveillance, wastewater surveillance

Recommended Citation

Estep, Gabrielle H.; Peer, Ashley; Salimi, Mohammad; Wiley, Michael; and Pratt, Catherine, "Using RT-PCR to Determine the Presence of Various Viruses Found in Nebraska Wastewater" (2022). Posters: 2022 Summer Undergraduate Research Program. 36.
https://digitalcommons.unmc.edu/surp2022/36