Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening.

Authors

Channabasavaiah B. Gurumurthy, University of Nebraska Medical CenterFollow
Poonam S. Joshi, University of Nebraska Medical CenterFollow
Scott G. Kurz, University of Nebraska Medical Center
Masato Ohtsuka, Tokai University School of Medicine
Rolen M. Quadros, University of Nebraska Medical CenterFollow
Donald W. Harms, University of Nebraska Medical CenterFollow
K.C. Kent Lloyd, University of California

Document Type

Article

Journal Title

International Journal of Genomics

Publication Date

1-1-2015

Volume

2015

Abstract

Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise. Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation. Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strains. The availability of well-established marker sets for speed congenic screens would enable the scientific community to transfer mutations across strain backgrounds. In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models. The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.

DOI Link

10.1155/2015/735845

ISSN

2314-436X

Creative Commons License

This work is licensed under a Creative Commons Attribution 3.0 License.

Recommended Citation

Gurumurthy, Channabasavaiah B.; Joshi, Poonam S.; Kurz, Scott G.; Ohtsuka, Masato; Quadros, Rolen M.; Harms, Donald W.; and Lloyd, K.C. Kent, "Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening." (2015). Journal Articles: Genetics, Cell Biology & Anatomy. 17.
https://digitalcommons.unmc.edu/com_gcba_articles/17