Document Type
Article
Journal Title
PLoS One
Publication Date
11-2013
Volume
8
Abstract
Genetic studies often use genomic DNA from whole blood cells, of which the majority are the polymorphonuclear myeloid cells. Those cells undergo dramatic change of nuclear morphology following cellular differentiation. It remains elusive if the nuclear morphological change accompanies sequence alternations from the intact genome. If such event exists, it will cause a serious problem in using such type of genomic DNA for genetic study as the sequences will not represent the intact genome in the host individuals. Using exome sequencing, we compared the coding regions between neutrophil, which is the major type of polymorphonuclear cells, and CD4+ T cell, which has an intact genome, from the same individual. The results show that exon sequences between the two cell types are essentially the same. The minor differences represented by the missed exons and base changes between the two cell types were validated to be mainly caused by experimental errors. Our study concludes that genomic DNA from whole blood cells can be safely used for genetic studies.
DOI Link
ISSN
1932-6203
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Xiao, Fengxia; Kim, Yeong C.; Wen, Hongxiu; Luo, Jiangtao; Xian Chen, Pei; Cowan, Kenneth; and Ming Wang, San, "The genome of polymorphonuclear neutrophils maintains normal coding sequences" (2013). Journal Articles: Genetics, Cell Biology & Anatomy. 3.
https://digitalcommons.unmc.edu/com_gcba_articles/3