Document Type
Article
Journal Title
BMC Genomics
Publication Date
2024
Volume
25
Abstract
BACKGROUND: Transgenic (Tg) mice are widely used in biomedical research, and they are typically generated by injecting transgenic DNA cassettes into pronuclei of one-cell stage zygotes. Such animals often show unreliable expression of the transgenic DNA, one of the major reasons for which is random insertion of the transgenes. We previously developed a method called "pronuclear injection-based targeted transgenesis" (PITT), in which DNA constructs are directed to insert at pre-designated genomic loci. PITT was achieved by pre-installing so called landing pad sequences (such as heterotypic LoxP sites or attP sites) to create seed mice and then injecting Cre recombinase or PhiC31 integrase mRNAs along with a compatible donor plasmid into zygotes derived from the seed mice. PITT and its subsequent version, improved PITT (i-PITT), overcome disadvantages of conventional Tg mice such as lack of consistent and reliable expression of the cassettes among different Tg mouse lines, and the PITT approach is superior in terms of cost and labor. One of the limitations of PITT, particularly using Cre-mRNA, is that the approach cannot be used for insertion of conditional expression cassettes using Cre-LoxP site-specific recombination. This is because the LoxP sites in the donor plasmids intended for achieving conditional expression of the transgene will interfere with the PITT recombination reaction with LoxP sites in the landing pad.
RESULTS: To enable the i-PITT method to insert a conditional expression cassette, we modified the approach by simultaneously using PhiC31o and FLPo mRNAs. We demonstrate the strategy by creating a model containing a conditional expression cassette at the Rosa26 locus with an efficiency of 13.7%. We also demonstrate that inclusion of FLPo mRNA excludes the insertion of vector backbones in the founder mice.
CONCLUSIONS: Simultaneous use of PhiC31 and FLP in i-PITT approach allows insertion of donor plasmids containing Cre-loxP-based conditional expression cassettes.
MeSH Headings
Animals, Mice, Mice, Transgenic, Integrases, Genome, Transgenes, Gene Targeting, Gene Transfer Techniques, Plasmids, RNA, Messenger, Mutagenesis, Insertional
DOI Link
ISSN
1471-2164
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Miura, Hiromi; Nakamura, Ayaka; Kurosaki, Aki; Kotani, Ai; Motojima, Masaru; Tanaka, Keiko; Kakuta, Shigeru; Ogiwara, Sanae; Ohmi, Yuhsuke; Komaba, Hirotaka; Schilit, Samantha L. P.; Morton, Cynthia C.; Gurumurthy, Channabasavaiah B.; and Ohtsuka, Masato, "Targeted Insertion of Conditional Expression Cassettes into the Mouse Genome Using the Modified i-PITT" (2024). Journal Articles: Genetics, Cell Biology & Anatomy. 57.
https://digitalcommons.unmc.edu/com_gcba_articles/57
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