Document Type
Article
Journal Title
Nutrients
Publication Date
Fall 11-27-2014
Volume
6
Abstract
Workers exposed to organic dusts from concentrated animal feeding operations (CAFOs) are at risk for developing airway inflammatory diseases. Available preventative and therapeutic measures for alleviating dust-induced lung disease are inadequate. Because omega-3 fatty acids can mitigate inflammatory processes, we aimed to determine whether nutritional supplementation with the omega-3 fatty acid docosahexaenoic acid (DHA) could reduce the airway inflammatory consequences of exposures to organic dust. Aqueous extracts of organic dusts from swine CAFOs (ODE) were utilized. In DHA-pretreated human bronchial epithelial cells, lung fibroblasts, monocyte cell cultures, and precision-cut murine lung slices, we found that DHA pretreatment dose-dependently decreased ODE-induced inflammatory cytokine production. To determine the in vivo significance of DHA, C57BL/6 mice were orally administered DHA for seven days prior to treatment with intranasal ODE or saline inhalations. Animals treated with 2 mg DHA demonstrated significant reductions in ODE-induced bronchial alveolar lavage neutrophil influx and pro-inflammatory cytokine/chemokine production compared to mice exposed to ODE alone. Collectively, these data demonstrate that DHA affects several lung cells to reduce the airway inflammatory response to organic dust exposures. Dietary supplementation with DHA may be an effective therapeutic strategy to reduce the airway inflammatory consequences in individuals exposed to agriculture dust environments.
MeSH Headings
Air Pollutants, Animals, Anti-Inflammatory Agents, Cell Line, Disease Models, Animal, Docosahexaenoic Acids, Dose-Response Relationship, Drug, Dust, Epithelial Cells, Fibroblasts, Humans, Inflammation Mediators, Inhalation Exposure, Intercellular Adhesion Molecule-1, Lung, Male, Mice, Inbred C57BL, Monocytes, Neutrophil Infiltration, Pneumonia, Swine
DOI Link
ISSN
2072-6643
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Nordgren, Tara M.; Friemel, Taylor D.; Heires, Art J.; Poole, Jill A.; Wyatt, Todd A.; and Romberger, Debra J., "The omega-3 fatty acid docosahexaenoic acid attenuates organic dust-induced airway inflammation." (2014). Journal Articles: Pulmonary & Critical Care Med. 1.
https://digitalcommons.unmc.edu/com_pulm_articles/1
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Allergy and Immunology Commons, Critical Care Commons, Respiratory System Commons, Respiratory Tract Diseases Commons