Document Type
Article
Journal Title
Respiratory Research
Publication Date
2008
Volume
9
Abstract
BACKGROUND: We have previously reported that low concentrations of cigarette smoke extract induce DNA damage without leading to apoptosis or necrosis in human bronchial epithelial cells (HBECs), and that IL-6/STAT3 signaling contributes to the cell survival. Since NF-kappaB is also involved in regulating apoptosis and cell survival, the current study was designed to investigate the role of NF-kappaB in mediating cell survival in response to cigarette smoke exposure in HBECs.
METHODS: Both the pharmacologic inhibitor of NF-kappaB, curcumin, and RNA interference targeting p65 were used to block NF-kappaB signaling in HBECs. Apoptosis and cell survival were then assessed by various methods including COMET assay, LIVE/DEAD Cytotoxicity/Viability assay and colony formation assay.
RESULTS: Cigarette smoke extract (CSE) caused DNA damage and cell cycle arrest in S phase without leading to apoptosis in HBECs as evidenced by TUNEL assay, COMET assay and DNA content assay. CSE stimulated NF-kappaB -DNA binding activity and up-regulated Bcl-XL protein in HBECs. Inhibition of NF-kappaB by the pharmacologic inhibitor curcumin (20 microM) or suppression of p65 by siRNA resulted in a significant increase in cell death in response to cigarette smoke exposure. Furthermore, cells lacking p65 were incapable of forming cellular colonies when these cells were exposed to CSE, while they behaved normally in the regular culture medium.
CONCLUSION: The current study demonstrates that CSE activates NF-kappaB and up-regulates Bcl-XL through NF-kB activation in HBECs, and that CSE induces cell death in cells lacking p65. These results suggest that activation of NF-kappaB regulates cell survival following DNA damage by cigarette smoke in human bronchial epithelial cells.
MeSH Headings
Bronchi, Cell Line, Cell Survival, Cells, Cultured, Epithelial Cells, Humans, NF-kappa B, Plant Extracts, Smoke, Tars, Nicotiana, Tobacco Smoke Pollution
DOI Link
ISSN
1465-993X
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Liu, Xiangde; Togo, Shinsaku; Al-Mugotir, Mona; Kim, Huijung; Fang, Qiuhong; Kobayashi, Tetsu; Wang, Xingqi; Mao, Lijun; Bitterman, Peter; and Rennard, Stephen I., "NF-kappaB Mediates the Survival of Human Bronchial Epithelial Cells Exposed to Cigarette Smoke Extract" (2008). Journal Articles: Pulmonary & Critical Care Med. 80.
https://digitalcommons.unmc.edu/com_pulm_articles/80