Graduation Date

Fall 12-18-2020

Document Type

Thesis

Degree Name

Master of Science (MS)

Programs

Medical Sciences Interdepartmental Area

First Advisor

Dr. Sundaralingam Premaraj

Second Advisor

Dr. Thomas Petro

Third Advisor

Dr. Aimin Peng

Abstract

The purpose of this study is to characterize the inflammatory cytokines that are expressed in peripheral blood mononuclear cells (PBMCs) and THP-1 monocytes exposed to Invisalign® aligner material in vitro. Invisalign® material was ground into particles, soaked in artificial saliva, and the eluate was collected. Commercially available cryopreserved pooled and individual human PBMCs and THP-1 monocytes were cultured with Invisalign® material eluate and positive (lipopolysaccharide) and negative controls. The supernatants were collected and used with a BioLegend LEGENDplex™ multi-analyte flow assay, a sandwich enzyme-linked immunosorbent assay (ELISA), to identify the expression of TNF-α, IL-6, IL-8, IL-10, IFN-γ, IL-1β, and IL-12p70. The results of this study show that Invisalign® material stimulates IL-8 expression in certain individual donor PBMCs and in THP-1 monocytes. Invisalign® material also stimulates low levels of TNF-α expression in pooled PBMCs, certain individual donor PBMCs and THP-1 cells. IL-6 was slightly increased in certain individual donor PBMCs. IL-1β was also slightly stimulated by Invisalign® material in THP-1 cells. IL-10, IL-12p70, IFN-γ, and IL-6 were not detected after stimulation with Invisalign® material in THP-1 monocytes. Invisalign ® material stimulated IL-8 expression across experiments, though not significantly, in individual donor PBMCs and THP-1 monocytes when profiled with a BioLegend LEGENDplex™ multi-analyte flow assay. IL-8 is a chemokine responsible for directing neutrophils to the site of inflammation.

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