Document Type
Article
Journal Title
Annals of Clinical and Translational Neurology
Publication Date
2018
Volume
5
Abstract
OBJECTIVE: Neuronal Ceroid Lipofuscinoses (NCL) are fatal inherited neurodegenerative diseases with established neuronal cell death and increased ceramide levels in brain, hence, a need for disease-modifying drug candidates, with potential to enhance growth, reduce apoptosis and lower ceramide in neuronal precursor PC12 cells and human NCL cell lines using enhanced flupirtine aromatic carbamate derivatives in vitro.
METHODS: Aromatic carbamate derivatives were tested by establishing growth curves under pro-apoptotic conditions and activity evaluated by trypan blue and JC-1 staining, as well as a drop in pro-apoptotic ceramide in neuronal precursor PC12 cells following siRNA knockdown of the
RESULTS: Retigabine, the benzyl-derivatized carbamate and an allyl carbamate derivative were neuroprotective in CLN3-defective PC12 cells and rescued CLN1-/CLN2-/CLN3-/CLN6-/CLN8 patient-derived lymphoblasts from diminished growth and accelerated apoptosis. All drugs decreased ceramide in CLN1-/CLN2-/CLN3-/CLN6-/CLN8 patient-derived lymphoblasts. Increased
INTERPRETATION: These findings establish that compounds analogous to flupirtine demonstrate anti-apoptotic activity with potential for treatment of NCL disease and use of ceramide as a marker for these diseases.
DOI Link
ISSN
2328-9503
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Makoukji, Joelle; Saadeh, Fadi; Mansour, Karl Albert; El-Sitt, Sally; Al Ali, Jamal; Kinarivala, Nihar; Trippier, Paul C.; and Boustany, Rose-Mary, "Flupirtine Derivatives as Potential Treatment for the Neuronal Ceroid Lipofuscinoses" (2018). Journal Articles: Pharmaceutical Sciences. 42.
https://digitalcommons.unmc.edu/cop_pharmsci_articles/42